%PDF-1.5 % 2 0 obj << /AcroForm << /Fields [] >> /Metadata 4 0 R /Names 5 0 R /OpenAction 6 0 R /Outlines 7 0 R /PageMode /None /Pages 8 0 R /Type /Catalog /ViewerPreferences << /FitWindow true >> >> endobj 4 0 obj << /Length 5596 /Subtype /XML /Type /Metadata >> stream 2016-01-29T17:36:35+08:00 LaTeX with hyperref package 2018-11-06T16:32:14+08:00 liensinine; neferine; cardiotoxicity; hiPS-CM; real-time analysis PDF-XChange Viewer [Version: 2.0 (Build 44.0) (Dec 30 2009; 19:40:12)] application/pdf liensinine neferine cardiotoxicity hiPS-CM real-time analysis Yangyang Yu Shennan Sun Shifeng Wang Qiao Zhang Ming Li Feng Lan Shiyou Li and Chunsheng Liu Due to drug-induced potential congestive heart failure and irreversible dilated cardiomyopathies, preclinical evaluation of cardiac dysfunction is important to assess the safety of traditional or novel treatments. The embryos of Nelumbo nucifera Gaertner seeds are a homology of traditional Chinese medicine and food. In this study, we applied the real time cellular analysis (RTCA) Cardio system, which can real-time monitor the contractility of cardiomyocytes (CMs), to evaluate drug safety in rat neonatal CMs and human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs). This study showed detailed biomechanical CM contractility in vitro, and provided insights into the cardiac dysfunctions associated with liensinine and neferine treatment. These effects exhibited dose and time-dependent recovery. Neferine showed stronger blocking effect in rat neonatal CMs than liensinine. In addition, the effects of liensinine and neferine were further evaluated on hiPS-CMs. Our study also indicated that both liensinine and neferine can cause disruption of calcium homeostasis. For the first time, we demonstrated the potential cardiac side effects of liensinine or neferine. While the same inhibition was observed on hiPS-CMs, more importantly, this study introduced an efficient and effective approach to evaluate the cardiotoxicity of the existing and novel drug candidates. Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes This is pdfTeX, Version 3.14159265-2.6-1.40.15 (TeX Live 2014/W32TeX) kpathsea version 6.2.0 uuid:d62e2f34-6b5b-4a9f-bec8-dd98d6b5579e uuid:94259fe6-d1df-46ee-bc1e-94a2c67eae90 endstream endobj 24 0 obj << /Filter [/FlateDecode] /Length 3213 >> stream xڝYY6~_GNvDiH^?ImA$5BL –nt?e)_[bb_\n&??R7E^_v~fri6xgVnFi1cced/?^ɳϷع